Journal article

Muscle Protein Synthesis after Protein Administration in Critical Illness

LAS Chapple, IWK Kouw, MJ Summers, LM Weinel, S Gluck, E Raith, P Slobodian, S Soenen, AM Deane, LJC van Loon, MJ Chapman

American Journal of Respiratory and Critical Care Medicine | AMER THORACIC SOC | Published : 2022

Abstract

Rationale: Dietary protein may attenuate the muscle atrophy experienced by patients in the ICU, yet protein handling is poorly understood. Objectives: To quantify protein digestion and amino acid absorption and fasting and postprandial myofibrillar protein synthesis during critical illness. Methods: Fifteen mechanically ventilated adults (12 male; aged 50 ± 17 yr; body mass index, 27 ± 5 kg⋅m-2) and 10 healthy control subjects (6 male; 54 ± 23 yr; body mass index, 27 ± 4 kg⋅m-2) received a primed intravenous L-[ring-2H5]-phenylalanine, L-[3,5-2H2]-tyrosine, and L-[1-13C]-leucine infusion over 9.5 hours and a duodenal bolus of intrinsically labeled (L-[1-13C]-phenylalanine and L-[1-13C]-leuci..

View full abstract

University of Melbourne Researchers

Grants

Awarded by National Health and Medical Research Council


Funding Acknowledgements

Supported by the National Health and Medical Research Council project grant APP1144496. L.-a.S.C. has been supported by a European Society for Clinical Nutrition and Metabolism Research Fellowship (2018) and a National Health and Medical Research Council Early Career Fellowship (2019-2022). The Royal Adelaide Hospital provided infrastructure and administrative support.